Superior Multiplexing Capacity of PlexPrimers Enables Sensitive and Specific Detection of SNPs and Clustered Mutations in qPCR

BACKGROUND: Whilst qPCR provides an extremely powerful tool for genetic analysis, some applications such as multiplexing variant alleles (eg SNPs, point mutations or deletions), remain challenging using current primer/probe systems. The novel design features of PlexPrimers allow sensitive, multiplex...

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Dettagli Bibliografici
Pubblicato in:PLoS One
Autori principali: Tan, Lit Yeen, Walker, Samantha Michelle, Lonergan, Tina, Lima, Nicole Elizabeth, Todd, Alison Velyian, Mokany, Elisa
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2017
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5256879/
https://ncbi.nlm.nih.gov/pubmed/28114309
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0170087
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