CRISPR‐Cas9‐mediated efficient directed mutagenesis and RAD51‐dependent and RAD51‐independent gene targeting in the moss Physcomitrella patens

The ability to address the CRISPR‐Cas9 nuclease complex to any target DNA using customizable single‐guide RNAs has now permitted genome engineering in many species. Here, we report its first successful use in a nonvascular plant, the moss Physcomitrella patens. Single‐guide RNAs (sgRNAs) were design...

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Veröffentlicht in:Plant Biotechnol J
Hauptverfasser: Collonnier, Cécile, Epert, Aline, Mara, Kostlend, Maclot, François, Guyon‐Debast, Anouchka, Charlot, Florence, White, Charles, Schaefer, Didier G., Nogué, Fabien
Format: Artigo
Sprache:Inglês
Veröffentlicht: John Wiley and Sons Inc. 2016
Schlagworte:
Research Articles
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5253467/
https://ncbi.nlm.nih.gov/pubmed/27368642
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/pbi.12596
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