CRISPR‐Cas9‐mediated efficient directed mutagenesis and RAD51‐dependent and RAD51‐independent gene targeting in the moss Physcomitrella patens

The ability to address the CRISPR‐Cas9 nuclease complex to any target DNA using customizable single‐guide RNAs has now permitted genome engineering in many species. Here, we report its first successful use in a nonvascular plant, the moss Physcomitrella patens. Single‐guide RNAs (sgRNAs) were design...

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Detalles Bibliográficos
Publicado en:Plant Biotechnol J
Main Authors: Collonnier, Cécile, Epert, Aline, Mara, Kostlend, Maclot, François, Guyon‐Debast, Anouchka, Charlot, Florence, White, Charles, Schaefer, Didier G., Nogué, Fabien
Formato: Artigo
Idioma:Inglês
Publicado: John Wiley and Sons Inc. 2016
Assuntos:
Research Articles
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5253467/
https://ncbi.nlm.nih.gov/pubmed/27368642
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/pbi.12596
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