An Efficient Electroporation Protocol for the Genetic Modification of Mammalian Cells

Genetic modification of cell lines and primary cells is an expensive and cumbersome approach, often involving the use of viral vectors. Electroporation using square-wave generating devices, like Lonza’s Nucleofector, is a widely used option, but the costs associated with the acquisition of electropo...

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Detalhes bibliográficos
Publicado no:Front Bioeng Biotechnol
Main Authors: Chicaybam, Leonardo, Barcelos, Camila, Peixoto, Barbara, Carneiro, Mayra, Limia, Cintia Gomez, Redondo, Patrícia, Lira, Carla, Paraguassú-Braga, Flávio, Vasconcelos, Zilton Farias Meira De, Barros, Luciana, Bonamino, Martin Hernán
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2017
Assuntos:
Bioengineering and Biotechnology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5253374/
https://ncbi.nlm.nih.gov/pubmed/28168187
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2016.00099
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