An Efficient Electroporation Protocol for the Genetic Modification of Mammalian Cells

Genetic modification of cell lines and primary cells is an expensive and cumbersome approach, often involving the use of viral vectors. Electroporation using square-wave generating devices, like Lonza’s Nucleofector, is a widely used option, but the costs associated with the acquisition of electropo...

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Vydáno v:Front Bioeng Biotechnol
Hlavní autoři: Chicaybam, Leonardo, Barcelos, Camila, Peixoto, Barbara, Carneiro, Mayra, Limia, Cintia Gomez, Redondo, Patrícia, Lira, Carla, Paraguassú-Braga, Flávio, Vasconcelos, Zilton Farias Meira De, Barros, Luciana, Bonamino, Martin Hernán
Médium: Artigo
Jazyk:Inglês
Vydáno: Frontiers Media S.A. 2017
Témata:
Bioengineering and Biotechnology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5253374/
https://ncbi.nlm.nih.gov/pubmed/28168187
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2016.00099
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