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Molecular Detection of Linezolid Resistance in Enterococcus faecium and Enterococcus faecalis by Use of 5′ Nuclease Real-Time PCR Compared to a Modified Classical Approach

A nucleotide transversion from guanine to uracil in the 23S rRNA confers linezolid resistance. We describe a real-time PCR using two Taqman probes that detects a single mutated allele among the genomes of Enterococcus faecium and Enterococcus faecalis. Results were confirmed by a classical approach...

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Udgivet i:J Clin Microbiol
Principais autores: Werner, Guido, Strommenger, Birgit, Klare, Ingo, Witte, Wolfgang
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2004
Fag:
Bacteriology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC525171/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15528734/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.42.11.5327-5331.2004
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