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cDNA cloning of human R-type pyruvate kinase and identification of a single amino acid substitution (Thr384----Met) affecting enzymatic stability in a pyruvate kinase variant (PK Tokyo) associated with hereditary hemolytic anemia.

cDNA clones for human R-type pyruvate kinase (PK) were isolated from a human reticulocyte cDNA library, constructed by PCR with a single gene-specific primer. The full-length cDNA was 2060 base pairs long, and the cDNA encoded 574 amino acids, the same number as that by rat R-type PK. Compared with...

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Bibliografski detalji
Glavni autori: Kanno, H, Fujii, H, Hirono, A, Miwa, S
Format: Artigo
Jezik:Inglês
Izdano: 1991
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC52478/
https://ncbi.nlm.nih.gov/pubmed/1896471
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