Oral Tolerance Induced by OVA Intake Ameliorates TNBS-Induced Colitis in Mice

INTRODUCTION: Literature data have shown that the consumption of dietary proteins may cause modulatory effects on the host immune system, process denominated oral tolerance by bystander suppression. It has been shown that the bystander suppression induced by dietary proteins can improve inflammatory...

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I publikationen:	PLoS One
Huvudupphovsmän:	Paiatto, Lisiery N., Silva, Fernanda G. D., Bier, Julia, Brochetto-Braga, Márcia R., Yamada, Áureo T., Tamashiro, Wirla M. S. C., Simioni, Patricia U.
Materialtyp:	Artigo
Språk:	Inglês
Publicerad:	Public Library of Science 2017
Ämnen:	Research Article
Länkar:	https://ncbi.nlm.nih.gov/pmc/articles/PMC5242488/ https://ncbi.nlm.nih.gov/pubmed/28099498 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0170205
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id	pubmed-5242488
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spelling	pubmed-52424882017-02-06 Oral Tolerance Induced by OVA Intake Ameliorates TNBS-Induced Colitis in Mice Paiatto, Lisiery N. Silva, Fernanda G. D. Bier, Julia Brochetto-Braga, Márcia R. Yamada, Áureo T. Tamashiro, Wirla M. S. C. Simioni, Patricia U. PLoS One Research Article INTRODUCTION: Literature data have shown that the consumption of dietary proteins may cause modulatory effects on the host immune system, process denominated oral tolerance by bystander suppression. It has been shown that the bystander suppression induced by dietary proteins can improve inflammatory diseases such as experimental arthritis. Here, we evaluated the effects of oral tolerance induced by ingestion of ovalbumin (OVA) on TNBS-induced colitis in mice, an experimental model for human Crohn’s disease. METHODS AND RESULTS: Colitis was induced in BALB/c mice by instilling a single dose of TNBS (100 mg/kg) in ethanol into the colon. Tolerized mice received OVA (4mg/mL) dissolved in the drinking water for seven consecutive days, prior to or concomitantly with the intrarectal instillation. Control groups received protein-free water and ethanol by intrarectal route. We observed that either the prior or concomitant induction of oral tolerance were able to reduce the severity of colitis as noted by recovery of body weight gain, improvement of clinical signs and reduction of histological abnormalities. The in vitro proliferation of spleen cells from tolerant colitic mice was lower than that of control mice, the same as the frequencies of CD4(+) T cells secreting IL-17 and IFN-γ. The frequencies of regulatory T cells and T cells secreting IL-10 have increased significantly in mice orally treated with OVA. The levels of inflammatory cytokines (IL-17A, TNF-α, IL-6 and IFN-γ) were lower in supernatants of cells from tolerant colitic mice, whereas IL-10 levels were higher. CONCLUSION: Our data show that the modulation of immune response induced by oral tolerance reduces the severity of experimental colitis. Such modulation may be partially attributed to the increase of Treg cells and reduction of pro-inflammatory cytokines in peripheral lymphoid organs of tolerant mice by bystander suppression. Public Library of Science 2017-01-18 /pmc/articles/PMC5242488/ /pubmed/28099498 http://dx.doi.org/10.1371/journal.pone.0170205 Text en © 2017 Paiatto et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
institution	US NLM
collection	PubMed Central
language	Inglês
format	Artigo
topic	Research Article
spellingShingle	Research Article Paiatto, Lisiery N. Silva, Fernanda G. D. Bier, Julia Brochetto-Braga, Márcia R. Yamada, Áureo T. Tamashiro, Wirla M. S. C. Simioni, Patricia U. Oral Tolerance Induced by OVA Intake Ameliorates TNBS-Induced Colitis in Mice
description	INTRODUCTION: Literature data have shown that the consumption of dietary proteins may cause modulatory effects on the host immune system, process denominated oral tolerance by bystander suppression. It has been shown that the bystander suppression induced by dietary proteins can improve inflammatory diseases such as experimental arthritis. Here, we evaluated the effects of oral tolerance induced by ingestion of ovalbumin (OVA) on TNBS-induced colitis in mice, an experimental model for human Crohn’s disease. METHODS AND RESULTS: Colitis was induced in BALB/c mice by instilling a single dose of TNBS (100 mg/kg) in ethanol into the colon. Tolerized mice received OVA (4mg/mL) dissolved in the drinking water for seven consecutive days, prior to or concomitantly with the intrarectal instillation. Control groups received protein-free water and ethanol by intrarectal route. We observed that either the prior or concomitant induction of oral tolerance were able to reduce the severity of colitis as noted by recovery of body weight gain, improvement of clinical signs and reduction of histological abnormalities. The in vitro proliferation of spleen cells from tolerant colitic mice was lower than that of control mice, the same as the frequencies of CD4(+) T cells secreting IL-17 and IFN-γ. The frequencies of regulatory T cells and T cells secreting IL-10 have increased significantly in mice orally treated with OVA. The levels of inflammatory cytokines (IL-17A, TNF-α, IL-6 and IFN-γ) were lower in supernatants of cells from tolerant colitic mice, whereas IL-10 levels were higher. CONCLUSION: Our data show that the modulation of immune response induced by oral tolerance reduces the severity of experimental colitis. Such modulation may be partially attributed to the increase of Treg cells and reduction of pro-inflammatory cytokines in peripheral lymphoid organs of tolerant mice by bystander suppression.
author	Paiatto, Lisiery N. Silva, Fernanda G. D. Bier, Julia Brochetto-Braga, Márcia R. Yamada, Áureo T. Tamashiro, Wirla M. S. C. Simioni, Patricia U.
author_facet	Paiatto, Lisiery N. Silva, Fernanda G. D. Bier, Julia Brochetto-Braga, Márcia R. Yamada, Áureo T. Tamashiro, Wirla M. S. C. Simioni, Patricia U.
author_sort	Paiatto, Lisiery N.
title	Oral Tolerance Induced by OVA Intake Ameliorates TNBS-Induced Colitis in Mice
title_short	Oral Tolerance Induced by OVA Intake Ameliorates TNBS-Induced Colitis in Mice
title_full	Oral Tolerance Induced by OVA Intake Ameliorates TNBS-Induced Colitis in Mice
title_fullStr	Oral Tolerance Induced by OVA Intake Ameliorates TNBS-Induced Colitis in Mice
title_full_unstemmed	Oral Tolerance Induced by OVA Intake Ameliorates TNBS-Induced Colitis in Mice
title_sort	oral tolerance induced by ova intake ameliorates tnbs-induced colitis in mice
publisher	Public Library of Science
container_title	PLoS One
publishDate	2017
url	https://ncbi.nlm.nih.gov/pmc/articles/PMC5242488/ https://ncbi.nlm.nih.gov/pubmed/28099498 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0170205
_version_	1818684604317433856

Oral Tolerance Induced by OVA Intake Ameliorates TNBS-Induced Colitis in Mice

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