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A method for rapidly screening functionality of actin mutants and tagged actins

Recombinant production and biochemical analysis of actin mutants has been hampered by the fact that actin has an absolute requirement for the eukaryotic chaperone CCT to reach its native state. We therefore have developed a method to rapidly screen the folding capacity and functionality of actin var...

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Hlavní autoři: Rommelaere, Heidi, Waterschoot, Davy, Neirynck, Katrien, Vandekerckhove, Joël, Ampe, Christophe
Médium: Artigo
Jazyk:Inglês
Vydáno: Biological Procedures Online 2004
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC524212/
https://ncbi.nlm.nih.gov/pubmed/15514698
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1251/bpo94
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