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Shifts in the fluorescence lifetime of EGFP during bacterial phagocytosis measured by phase-sensitive flow cytometry

Phase-sensitive flow cytometry (PSFC) is a technique in which fluorescence excited state decay times are measured as fluorescently labeled cells rapidly transit a finely focused, frequency-modulated laser beam. With PSFC the fluorescence lifetime is taken as a cytometric parameter to differentiate i...

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Detalles Bibliográficos
Publicado en:Sci Rep
Main Authors: Li, Wenyan, Houston, Kevin D., Houston, Jessica P.
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5238435/
https://ncbi.nlm.nih.gov/pubmed/28091553
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep40341
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