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Directly labeled DNA probes using fluorescent nucleotides with different length linkers.
Directly labeled fluorescent DNA probes have been made by nick translation and PCR using dUTP attached to the fluorescent label, Cy3, with different length linkers. With preparation of probes by PCR we find that linker length affects the efficiency of incorporation of Cy3-dUTP, the yield of labeled...
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| 主要な著者: | , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
1994
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| オンライン・アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC523738/ https://ncbi.nlm.nih.gov/pubmed/8078779 |
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