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Construction of Gpm6a/Reelin(GFPCreERT2) by BAC recombination using a specific gene in hepatic mesothelial or stellate cells
AIM: To prepare a Gpm6a/Reelin(GFPCreERT2) construct with a rapid and reliable strategy using a bacterial artificial chromosome (BAC). METHODS: Gpm6a and Reelin BACs were purified and transformed into SW102 E. coli by electroporation. The GFPCreERT2 fragment was prepared from a shuttle vector and tr...
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| Publicado en: | World J Gastroenterol |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Baishideng Publishing Group Inc
2017
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5236502/ https://ncbi.nlm.nih.gov/pubmed/28127196 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v23.i2.224 |
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