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Construction of Gpm6a/Reelin(GFPCreERT2) by BAC recombination using a specific gene in hepatic mesothelial or stellate cells

AIM: To prepare a Gpm6a/Reelin(GFPCreERT2) construct with a rapid and reliable strategy using a bacterial artificial chromosome (BAC). METHODS: Gpm6a and Reelin BACs were purified and transformed into SW102 E. coli by electroporation. The GFPCreERT2 fragment was prepared from a shuttle vector and tr...

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Bibliographische Detailangaben
Veröffentlicht in:World J Gastroenterol
Hauptverfasser: Shi, Hong-Bo, Lou, Jin-Li, Shi, Hong-Lin, Ren, Feng, Chen, Yu, Duan, Zhong-Ping
Format: Artigo
Sprache:Inglês
Veröffentlicht: Baishideng Publishing Group Inc 2017
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5236502/
https://ncbi.nlm.nih.gov/pubmed/28127196
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v23.i2.224
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