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Bead-shift isolation of protein--DNA complexes on a 5S RNA gene.

Specific protein-DNA complexes formed on a Xenopus 5S RNA gene were isolated and characterized using a novel technique. A DNA template reversibly immobilized on paramagnetic beads was used to capture, affinity purify, and concentrate protein--DNA complexes formed in a whole cell extract. The complex...

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Autors principals: Peck, L J, Bartilson, M, DeRisi, J L
Format: Artigo
Idioma:Inglês
Publicat: 1994
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC523601/
https://ncbi.nlm.nih.gov/pubmed/8127682
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