Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae

We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones...

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Autors principals: Boyer, Jeanne, Badis, Gwenaël, Fairhead, Cécile, Talla, Emmanuel, Hantraye, Florence, Fabre, Emmanuelle, Fischer, Gilles, Hennequin, Christophe, Koszul, Romain, Lafontaine, Ingrid, Ozier-Kalogeropoulos, Odile, Ricchetti, Miria, Richard, Guy-Franck, Thierry, Agnès, Dujon, Bernard
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2004
Matèries:
Method
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC522879/
https://ncbi.nlm.nih.gov/pubmed/15345056
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/gb-2004-5-9-r72
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