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Characterization of Glycan Structures of Chondroitin Sulfate-Glycopeptides Facilitated by Sodium Ion-Pairing and Positive Mode LC-MS/MS

Purification and liquid chromatography-tandem mass spectrometry (LC-MS/MS) characterization of glycopeptides, originating from protease digests of glycoproteins, enables site-specific analysis of protein N- and O-glycosylations. We have described a protocol to enrich, hydrolyze by chondroitinase ABC...

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Detalhes bibliográficos
Publicado no:J Am Soc Mass Spectrom
Main Authors: Nilsson, Jonas, Noborn, Fredrik, Gomez Toledo, Alejandro, Nasir, Waqas, Sihlbom, Carina, Larson, Göran
Formato: Artigo
Idioma:Inglês
Publicado em: Springer US 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5227003/
https://ncbi.nlm.nih.gov/pubmed/27873218
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13361-016-1539-1
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