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High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Nanoliter scale real-time PCR uses spatial multiplexing to allow multiple assays to be run in parallel on a single plate without the typical drawbacks of combining reactions together. We designed and evaluated a panel based on this principle to rapidly identify the presence of common disease agents...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Goodman, Laura B., Anderson, Renee R., Slater, Marcia, Ortenberg, Elen, Renshaw, Randall W., Chilson, Brittany D., Laverack, Melissa A., Beeby, John S., Dubovi, Edward J., Glaser, Amy L.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5226323/
https://ncbi.nlm.nih.gov/pubmed/27929456
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54781
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