Rapid Production of Gene Replacement Constructs and Generation of a Green Fluorescent Protein-Tagged Centromeric Marker in Aspergillus nidulans

A method to rapidly generate gene replacement constructs by fusion PCR is described for Aspergillus nidulans. The utility of the approach is demonstrated by green fluorescent protein (GFP) tagging of A. nidulans ndc80 to visualize centromeres through the cell cycle. The methodology makes possible la...

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Main Authors: Yang, Lin, Ukil, Leena, Osmani, Aysha, Nahm, Francis, Davies, Jonathan, De Souza, Colin P. C., Dou, Xiaowei, Perez-Balaguer, Ariadna, Osmani, Stephen A.
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2004
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Articles
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC522605/
https://ncbi.nlm.nih.gov/pubmed/15470263
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/EC.3.5.1359-1362.2004
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