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Combining Ca2+ imaging with -glutamate photorelease
We describe simple configurations and methods to measure optical Ca(2+) signals in response to photorelease of L-glutamate. This photostimulation allows activation of postsynaptic glutamate receptors without activation of voltage-gated Ca(2+) channels permitting the separation and the analysis of di...
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| Udgivet i: | Cold Spring Harb Protoc |
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| Main Authors: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Cold Spring Harbor Laboratory Press
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5225263/ https://ncbi.nlm.nih.gov/pubmed/24298028 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot073122 |
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