Multiplex Detection of Mutations in Clinical Isolates of Rifampin-Resistant Mycobacterium tuberculosis by Short Oligonucleotide Ligation Assay on DNA Chips

A new approach, short-oligonucleotide-ligation assay on DNA chip (SOLAC), is developed to detect mutations in rifampin-resistant Mycobacterium tuberculosis. The method needs only four common probes to detect 15 mutational variants of the rpoB gene within 12 h. Fifty-five rifampin-resistant M. tuberc...

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Bibliografiske detaljer
Main Authors: Deng, Jiao-Yu, Zhang, Xian-En, Lu, Hong-Bing, Liu, Qiang, Zhang, Zhi-Ping, Zhou, Ya-Feng, Xie, Wei-Hong, Fu, Zhi-Jun
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2004
Fag:
Mycobacteriology and Aerobic Actinomycetes
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC522320/
https://ncbi.nlm.nih.gov/pubmed/15472360
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.10.4850-4852.2004
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