Reverse Transcription-Booster PCR for Detection of Noroviruses in Shellfish

The methods commonly used for norovirus (NV) detection are based on reverse transcription-PCR (RT-PCR) followed by confirmation of the amplified sequence. To increase sensitivity, an RT-booster PCR was developed. The proposed method showed an increase in sensitivity at least 2 log units for all the...

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Detalles Bibliográficos
Publicado en:Appl Environ Microbiol
Autores principales: De Medici, Dario, Croci, Luciana, Suffredini, Elisabetta, Toti, Laura
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology (ASM) 2004
Materias:
Methods
Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC522127/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15466586/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.10.6329-6332.2004
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