Reverse Transcription-Booster PCR for Detection of Noroviruses in Shellfish

The methods commonly used for norovirus (NV) detection are based on reverse transcription-PCR (RT-PCR) followed by confirmation of the amplified sequence. To increase sensitivity, an RT-booster PCR was developed. The proposed method showed an increase in sensitivity at least 2 log units for all the...

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Detalhes bibliográficos
Main Authors: De Medici, Dario, Croci, Luciana, Suffredini, Elisabetta, Toti, Laura
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC522127/
https://ncbi.nlm.nih.gov/pubmed/15466586
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.10.6329-6332.2004
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