Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system.
A method is described for measuring cytosolic free Ca2+ and its time-dependent changes in the yeast Saccharomyces cerevisiae by using the luminescent protein aequorin as a Ca(2+)-specific indicator. This method with intact yeast cells is labeled "in vivo" to distinguish it from methods with cell ext...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1991
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC52192/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1862111/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.15.6878 |
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