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Detection of Isoforms Differing by a Single-charge Unit in Individual Cells
To measure protein isoforms in individual mammalian cells we report single-cell resolution isoelectric focusing (scIEF) and high-selectivity immunoprobing. Microfluidic design and photoactivatable materials establish the tunable pH gradients required by IEF and precisely control transport and handli...
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| Udgivet i: | Angew Chem Int Ed Engl |
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| Main Authors: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2016
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5201312/ https://ncbi.nlm.nih.gov/pubmed/27595864 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201606039 |
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