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Monitoring Chemokine Receptor Trafficking by Confocal Immunofluorescence Microscopy
Here, we describe a protocol to detect chemokine receptor CXCR4 by confocal immunofluorescence microscopy in HeLa cells treated with its chemokine ligand CXCL12. Typically, ligand-activated chemokine receptors undergo a multistep process of desensitization and/or internalization from the plasma memb...
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| Publicado no: | Methods Enzymol |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5201001/ https://ncbi.nlm.nih.gov/pubmed/26921951 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2015.10.004 |
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