Analysis of Mammalian Cell Proliferation and Macromolecule Synthesis Using Deuterated Water and Gas Chromatography-Mass Spectrometry

Deuterated water ((2)H(2)O), a stable isotopic tracer, provides a convenient and reliable way to label multiple cellular biomass components (macromolecules), thus permitting the calculation of their synthesis rates. Here, we have combined (2)H(2)O labelling, GC-MS analysis and a novel cell fractiona...

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Publicado no:Metabolites
Main Authors: Foletta, Victoria C., Palmieri, Michelle, Kloehn, Joachim, Mason, Shaun, Previs, Stephen F., McConville, Malcolm J., Sieber, Oliver M., Bruce, Clinton R., Kowalski, Greg M.
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2016
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5192440/
https://ncbi.nlm.nih.gov/pubmed/27754354
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/metabo6040034
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