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Intracellular Macromolecular Mobility Measured by Fluorescence Recovery after Photobleaching with Confocal Laser Scanning Microscopes

Fluorescence recovery after photobleaching (FRAP) is a widely used tool for estimating mobility parameters of fluorescently tagged molecules in cells. Despite the widespread use of confocal laser scanning microscopes (CLSMs) to perform photobleaching experiments, quantitative data analysis has been...

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Main Authors: Braga, José, Desterro, Joana M.P., Carmo-Fonseca, Maria
Format: Artigo
Jezik:Inglês
Izdano: The American Society for Cell Biology 2004
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC519164/
https://ncbi.nlm.nih.gov/pubmed/15292455
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E04-06-0496
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