A Tn3 derivative that can be used to make short in-frame insertions within genes.

A Tn3 derivative was constructed to make small in-frame insertions within genes. The transposon contains the URA3 gene, the tetA gene, a truncated lacZ, and phage P1 loxP recombination sites at either end. Insertions that have fused lacZ to an open reading frame are lac+ because they express the tru...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Hoekstra, M F, Burbee, D, Singer, J, Mull, E, Chiao, E, Heffron, F
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC51892/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1647034/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.12.5457
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