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Improved peptide identification in proteomics by two consecutive stages of mass spectrometric fragmentation

MS-based proteomics usually involves the fragmentation of tryptic peptides (tandem MS or MS(2)) and their identification by searching protein sequence databases. In ion trap instruments fragments can be further fragmented and analyzed, a process termed MS/MS/MS or MS(3). Here, we report that efficie...

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Detalhes bibliográficos
Main Authors: Olsen, Jesper V., Mann, Matthias
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2004
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC518757/
https://ncbi.nlm.nih.gov/pubmed/15347803
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0405549101
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