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Post-crystallization improvement of RNA crystal diffraction quality
The crystallization and structural determination of large RNAs and their complexes remain major bottlenecks in the mechanistic analysis of cellular and viral RNAs. Here, we describe a protocol that combines post-crystallization dehydration and ion replacement that dramatically improved the diffracti...
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| Publicado no: | Methods Mol Biol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5184826/ https://ncbi.nlm.nih.gov/pubmed/25967049 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2730-2_2 |
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