Development of a highly efficient expression cDNA cloning system: application to oncogene isolation.

We developed an expression cDNA cloning system capable of generating high-complexity libraries with unidirectionally inserted cDNA fragments and allowing efficient plasmid rescue. As an application of this system, a cDNA library was constructed from an NIH 3T3 transformant induced by mouse hepatocel...

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Hauptverfasser: Miki, T, Fleming, T P, Crescenzi, M, Molloy, C J, Blam, S B, Reynolds, S H, Aaronson, S A
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1991
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC51833/
https://ncbi.nlm.nih.gov/pubmed/2052597
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