Development of a highly efficient expression cDNA cloning system: application to oncogene isolation.

We developed an expression cDNA cloning system capable of generating high-complexity libraries with unidirectionally inserted cDNA fragments and allowing efficient plasmid rescue. As an application of this system, a cDNA library was constructed from an NIH 3T3 transformant induced by mouse hepatocel...

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Detalles Bibliográficos

Publicado en:	Proc Natl Acad Sci U S A
Principais autores:	Miki, T, Fleming, T P, Crescenzi, M, Molloy, C J, Blam, S B, Reynolds, S H, Aaronson, S A
Formato:	Artigo
Idioma:	Inglês
Publicado:	National Academy of Sciences 1991
Assuntos:	Research Article
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC51833/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2052597/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.12.5167
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