Highly Efficient CRISPR/Cas9-Mediated Homologous Recombination Promotes the Rapid Generation of Bacterial Artificial Chromosomes of Pseudorabies Virus

Bacterial artificial chromosomes (BACs) are powerful tools for the manipulation of the large genomes of DNA viruses, such as herpesviruses. However, the methods currently used to construct the recombinant viruses, an important intermediate link in the generation of BACs, involve the laborious proces...

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Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Guo, Jin-Chao, Tang, Yan-Dong, Zhao, Kuan, Wang, Tong-Yun, Liu, Ji-Ting, Gao, Jia-Cong, Chang, Xiao-Bo, Cui, Hong-Yu, Tian, Zhi-Jun, Cai, Xue-Hui, An, Tong-Qing
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2016
Assuntos:
Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5179515/
https://ncbi.nlm.nih.gov/pubmed/28066407
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2016.02110
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