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Fusion of green fluorescent protein to the C-terminus of granulysin alters its intracellular localization in comparison to the native molecule
The engineering of green fluorescent protein (GFP) fusion constructs in order to visibly tag a protein of interest has become a commonly used cell biology technique. Although caveats to this approach are obvious, literature reports in which the chimeric molecule behaves differently than the native m...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC517947/ https://ncbi.nlm.nih.gov/pubmed/15361253 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1477-5751-3-2 |
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