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Development of a Novel Chloramphenicol Resistance Expression Plasmid Used for Genetic Complementation of a fliG Deletion Mutant in Treponema denticola
A new expression plasmid containing the fla operon promoter and a staphylococcal chloramphenicol resistance gene, was constructed to help assess the role of fliG in Treponema denticola motility. Deletion of fliG resulted in a nonmotile mutant with a markedly decreased number of flagellar filaments....
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| Main Authors: | , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology
2004
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC517452/ https://ncbi.nlm.nih.gov/pubmed/15322052 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.72.9.5493-5497.2004 |
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