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Development of a Novel Chloramphenicol Resistance Expression Plasmid Used for Genetic Complementation of a fliG Deletion Mutant in Treponema denticola

A new expression plasmid containing the fla operon promoter and a staphylococcal chloramphenicol resistance gene, was constructed to help assess the role of fliG in Treponema denticola motility. Deletion of fliG resulted in a nonmotile mutant with a markedly decreased number of flagellar filaments....

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Main Authors: Slivienski-Gebhardt, Linda L., Izard, Jacques, Samsonoff, William A., Limberger, Ronald J.
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2004
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC517452/
https://ncbi.nlm.nih.gov/pubmed/15322052
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.72.9.5493-5497.2004
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