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Functional enrichment by direct plasmid recovery after Fluorescence Activated Cell Sorting
Iterative screening of expressed protein libraries using fluorescence-activated cell sorting (FACS) typically involves culturing the pooled clones after each sort. In these experiments, if cell viability is compromised by the sort conditions and/or expression of the target protein(s), rescue PCR pro...
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| Publicado no: | Biotechniques |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5173293/ https://ncbi.nlm.nih.gov/pubmed/26345509 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114329 |
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