Rapid Generation of Marker-Free P. falciparum Fluorescent Reporter Lines Using Modified CRISPR/Cas9 Constructs and Selection Protocol

The CRISPR/Cas9 system is a powerful genome editing technique employed in a wide variety of organisms including recently the human malaria parasite, P. falciparum. Here we report on further improvements to the CRISPR/Cas9 transfection constructs and selection protocol to more rapidly modify the P. f...

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Bibliographic Details
Published in:PLoS One
Main Authors: Mogollon, Catherin Marin, van Pul, Fiona J. A., Imai, Takashi, Ramesar, Jai, Chevalley-Maurel, Séverine, de Roo, Guido M., Veld, Sabrina A. J., Kroeze, Hans, Franke-Fayard, Blandine M. D., Janse, Chris J., Khan, Shahid M.
Format: Artigo
Language:Inglês
Published: Public Library of Science 2016
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5172577/
https://ncbi.nlm.nih.gov/pubmed/27997583
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0168362
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