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In vitro transcription of baculovirus immediate early genes: accurate mRNA initiation by nuclear extracts from both insect and human cells.

The production and characterization of nuclear extracts from uninfected Spodoptera frugiperda cells, capable of accurately initiating transcription of baculovirus immediate early genes in vitro, are described. Optimal in vitro transcription was dependent on the presence of a TATA box promoter elemen...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Hoopes, R R, Rohrmann, G F
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1991
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC51691/
https://ncbi.nlm.nih.gov/pubmed/2034691
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