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A facile method for expression and purification of (15)N isotope-labeled human Alzheimer's β-amyloid peptides from E. coli for NMR-based structural analysis

Alzheimer's disease (AD) is a progressive neurodegenerative disease affecting millions of people worldwide. AD is characterized by the presence of extracellular plaques composed of aggregated/oligomerized β-amyloid peptides with Aβ42 peptide representing a major isoform in the senile plaques. G...

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Bibliographische Detailangaben
Veröffentlicht in:Protein Expr Purif
Hauptverfasser: Sharma, Sudhir C., Armand, Tara, Ball, K. Aurelia, Chen, Anna, Pelton, Jeffrey G., Wemmer, David E., Head-Gordon, Teresa
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2015
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5161032/
https://ncbi.nlm.nih.gov/pubmed/26231074
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2015.07.012
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