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Switching substrate preference of thermophilic xylose isomerase from D-xylose to D-glucose by redesigning the substrate binding pocket.

The substrate specificity of thermophilic xylose isomerase from Clostridium thermosulfurogenes was examined by using predictions from the known crystal structure of the Arthrobacter enzyme and site-directed mutagenesis of the thermophile xylA gene. The orientation of glucose as a substrate in the ac...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Meng, M, Lee, C, Bagdasarian, M, Zeikus, J G
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1991
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC51584/
https://ncbi.nlm.nih.gov/pubmed/2023950
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