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Off Target, but Sequence-Specific, shRNA-Associated Trans-Activation of Promoter Reporters in Transient Transfection Assays

Transient transfection promoter reporter assays are commonly used in the study of transcriptional regulation, and can be used to define and characterize both cis-acting regulatory sequences and trans-acting factors. In the process of using a variety of reporter assays designed to study regulation of...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:PLoS One
Hauptverfasser: Masuda, Tomohiro, Wan, Jun, Yerrabelli, Anitha, Berlinicke, Cindy, Kallman, Alyssa, Qian, Jiang, Zack, Donald J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2016
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5158200/
https://ncbi.nlm.nih.gov/pubmed/27977714
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0167867
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