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In vitro system that synthesizes circular viral DNA of bacteriophage phi X 174.

An extract prepared from Escherichia coli cells infected with phi chi 174 bacteriophage was capable of incorporating dTTP into phage-specific DNAs in vitro. The synthesized DNAs were associated with proteins and sedimented with S values of 20, 50, and 90 in a sucrose gradient sedimentation. DNA isol...

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Bibliographic Details
Main Authors: Mukai, R, Hayashi, M
Format: Artigo
Language:Inglês
Published: 1977
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC515760/
https://ncbi.nlm.nih.gov/pubmed/327087
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