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Propidium Monoazide Integrated with qPCR Enables the Detection and Enumeration of Infectious Enteric RNA and DNA Viruses in Clam and Fermented Sausages

The increase of foodborne viral outbreaks highlights the need for a rapid and sensitive method for the prediction of viral infectivity in food samples. This study assesses the use of propidium monoazide (PMA) coupled with real-time PCR methods (RT-qPCR or qPCR for RNA or DNA viruses, respectively) i...

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Détails bibliographiques
Publié dans:Front Microbiol
Auteurs principaux: Quijada, Narciso M., Fongaro, Gislaine, Barardi, Célia R. M., Hernández, Marta, Rodríguez-Lázaro, David
Format: Artigo
Langue:Inglês
Publié: Frontiers Media S.A. 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5156952/
https://ncbi.nlm.nih.gov/pubmed/28018329
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2016.02008
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