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Fractionation of human spermatogenic cells using STA-PUT gravity sedimentation and their miRNA profiling
Human spermatogenic cells have not yet been isolated, and notably, their global miRNA profiles remain unknown. Here we have effectively isolated human spermatogonia, pachytene spermatocytes and round spermatids using STA-PUT velocity sedimentation. RT-PCR, immunocytochemistry and meiosis spread assa...
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| Publicado en: | Sci Rep |
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| Autores principales: | , , , , , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Nature Publishing Group
2015
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5155379/ https://ncbi.nlm.nih.gov/pubmed/25634318 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep08084 |
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