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Fractionation of human spermatogenic cells using STA-PUT gravity sedimentation and their miRNA profiling
Human spermatogenic cells have not yet been isolated, and notably, their global miRNA profiles remain unknown. Here we have effectively isolated human spermatogonia, pachytene spermatocytes and round spermatids using STA-PUT velocity sedimentation. RT-PCR, immunocytochemistry and meiosis spread assa...
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| Publicado no: | Sci Rep |
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| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5155379/ https://ncbi.nlm.nih.gov/pubmed/25634318 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep08084 |
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