Fractionation of human spermatogenic cells using STA-PUT gravity sedimentation and their miRNA profiling

Human spermatogenic cells have not yet been isolated, and notably, their global miRNA profiles remain unknown. Here we have effectively isolated human spermatogonia, pachytene spermatocytes and round spermatids using STA-PUT velocity sedimentation. RT-PCR, immunocytochemistry and meiosis spread assa...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Sci Rep
Hlavní autoři: Liu, Yun, Niu, Minghui, Yao, Chencheng, Hai, Yanan, Yuan, Qingqing, Liu, Yang, Guo, Ying, Li, Zheng, He, Zuping
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group 2015
Témata:
Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5155379/
https://ncbi.nlm.nih.gov/pubmed/25634318
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep08084
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!

Podobné jednotky