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Design and evaluation of primers targeting genes encoding NO-forming nitrite reductases: implications for ecological inference of denitrifying communities

The detection of NO-forming nitrite reductase genes (nir) has become the standard when studying denitrifying communities in the environment, despite well-known amplification biases in available primers. We review the performance of 35 published and 121 newly designed primers targeting the nirS and n...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Bonilla-Rosso, Germán, Wittorf, Lea, Jones, Christopher M., Hallin, Sara
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5155301/
https://ncbi.nlm.nih.gov/pubmed/27966627
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep39208
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