A faster way to make GFP-based biosensors: Two new transposons for creating multicolored libraries of fluorescent fusion proteins

BACKGROUND: There are now several ways to generate fluorescent fusion proteins by randomly inserting DNA encoding the Green Fluorescent Protein (GFP) into another protein's coding sequence. These approaches can be used to map regions in a protein that are permissive for GFP insertion or to create no...

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Veröffentlicht in:	BMC Biotechnol
Hauptverfasser:	Sheridan, Douglas L, Hughes, Thomas E
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	BMC 2004
Schlagworte:	Methodology Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC514711/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15317651/ https://ncbi.nlm.nih.govhttps://doi.org/10.1186/1472-6750-4-17
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