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A uniform procedure for the purification of CDK7/CycH/MAT1, CDK8/CycC and CDK9/CycT1

We have established a uniform procedure for the expression and purification of the cyclin-dependent kinases CDK7/CycH/MAT1, CDK8/CycC and CDK9/CycT1. We attach a His(6)-tag to one of the subunits of each complex and then co-express it together with the other subunits in Spodoptera frugiperda insect...

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Publicat a:Biol Proced Online
Autors principals: Pinhero, Reena, Liaw, Peter, Yankulov, Krassimir
Format: Artigo
Idioma:Inglês
Publicat: BMC 2004
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC514536/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15328539/
https://ncbi.nlm.nih.govhttps://doi.org/10.1251/bpo86
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