Transposon Express, a software application to report the identity of insertions obtained by comprehensive transposon mutagenesis of sequenced genomes: analysis of the preference for in vitro Tn5 transposition into GC-rich DNA

Comprehensive mutant libraries can be readily constructed by transposon mutagenesis. To systematically mutagenise the genome of the Gram-positive bacterium Streptomyces coelicolor A3(2), we have employed high-throughput shuttle transposon mutagenesis of a cosmid library prepared in Escherichia coli....

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Herron, Paul R., Hughes, Gareth, Chandra, Govind, Fielding, Susan, Dyson, Paul J.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2004
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC514396/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15308758/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh112
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