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Two-color fluorescence in situ hybridization using chromogenic substrates in Xenopus
Xenopus embryo yolk proteins present a serious barrier to fluorescence microscopy. Previously, in situ assays of gene transcripts in whole embryos was limited to the use of chromogenic alkaline phosphatase substrates, which restricted researchers’ ability to gauge coexpression of transcripts. Here,...
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| Pubblicato in: | Biotechniques |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2016
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5142608/ https://ncbi.nlm.nih.gov/pubmed/27839512 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114475 |
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