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Two-color fluorescence in situ hybridization using chromogenic substrates in Xenopus

Xenopus embryo yolk proteins present a serious barrier to fluorescence microscopy. Previously, in situ assays of gene transcripts in whole embryos was limited to the use of chromogenic alkaline phosphatase substrates, which restricted researchers’ ability to gauge coexpression of transcripts. Here,...

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Detalhes bibliográficos
Publicado no:Biotechniques
Main Authors: Agricola, Zachary, Cha, Sang-Wook
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5142608/
https://ncbi.nlm.nih.gov/pubmed/27839512
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114475
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