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Two-color fluorescence in situ hybridization using chromogenic substrates in Xenopus

Xenopus embryo yolk proteins present a serious barrier to fluorescence microscopy. Previously, in situ assays of gene transcripts in whole embryos was limited to the use of chromogenic alkaline phosphatase substrates, which restricted researchers’ ability to gauge coexpression of transcripts. Here,...

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Bibliografski detalji
Izdano u:Biotechniques
Glavni autori: Agricola, Zachary, Cha, Sang-Wook
Format: Artigo
Jezik:Inglês
Izdano: 2016
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5142608/
https://ncbi.nlm.nih.gov/pubmed/27839512
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114475
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