Overcoming a hemihedral twinning problem in tetrahydrofolate-dependent O-demethylase crystals by the microseeding method

A tetrahydrofolate-dependent O-demethylase, LigM, from Sphingobium sp. SYK-6 was crystallized by the hanging-drop vapour-diffusion method. However, the obtained P3(1)21 or P3(2)21 crystals, which diffracted to 2.5–3.3 Å resolution, were hemihedrally twinned. To overcome the twinning problem, microse...

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Bibliografske podrobnosti
izdano v:Acta Crystallogr F Struct Biol Commun
Main Authors: Harada, Ayaka, Sato, Yukari, Kamimura, Naofumi, Venugopalan, Nagarajan, Masai, Eiji, Senda, Toshiya
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2016
Teme:
Research Communications
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5137467/
https://ncbi.nlm.nih.gov/pubmed/27917838
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X16018665
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