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Direct identification of antibiotic resistance genes on single plasmid molecules using CRISPR/Cas9 in combination with optical DNA mapping

Bacterial plasmids are extensively involved in the rapid global spread of antibiotic resistance. We here present an assay, based on optical DNA mapping of single plasmids in nanofluidic channels, which provides detailed information about the plasmids present in a bacterial isolate. In a single exper...

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Bibliografski detalji
Izdano u:Sci Rep
Glavni autori: Müller, Vilhelm, Rajer, Fredrika, Frykholm, Karolin, Nyberg, Lena K., Quaderi, Saair, Fritzsche, Joachim, Kristiansson, Erik, Ambjörnsson, Tobias, Sandegren, Linus, Westerlund, Fredrik
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group 2016
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Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5131345/
https://ncbi.nlm.nih.gov/pubmed/27905467
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep37938
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