Making standards for quantitative real-time pneumococcal PCR

Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1–1.2). Genome-copies were less variable...

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Detalles Bibliográficos
Publicado en:Biomol Detect Quantif
Autores principales: Morpeth, Susan C., Huggett, Jim F., Murdoch, David R., Scott, J. Anthony G.
Formato: Artigo
Lenguaje:Inglês
Publicado: Elsevier 2014
Materias:
Short Communication
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5121202/
https://ncbi.nlm.nih.gov/pubmed/27896138
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bdq.2014.11.003
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