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A Solution to the Common Problem of the Synthesis and Applications of Hexachlorofluorescein Labeled Oligonucleotides

A common problem of the preparation of hexachlorofluorescein labeled oligonucleotides is the transformation of the fluorophore to an arylacridine derivative under standard ammonolysis conditions. We show here that the arylacridine byproduct with distinct optical characteristics cannot be efficiently...

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Bibliografische gegevens
Gepubliceerd in:PLoS One
Hoofdauteurs: Chuvilin, Andrey N., Smirnov, Igor P., Mosina, Alena G., Varizhuk, Anna M., Pozmogova, Galina E.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Public Library of Science 2016
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5115841/
https://ncbi.nlm.nih.gov/pubmed/27861573
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0166911
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